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Imaging aquisition by confocal microscopy

Confocal microscopy on live or fixed biological samples, from different origins. Specialized technical training.

Confocal point-scanning microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is based on an upright microscope body, with motorized XY stage, and has live imaging facilities (temperature control and perfusion system). Applications include for example time-lapse imaging, photobleaching experiments, colocalization studies, spectral detection and 3D imaging.

Techniques:
  • Fluorescence imaging with optical seccioning
  • Live cell imaging
  • Sample preparation for microscopy
  • Imaging of live tissue sections
  • Colocalisation assays
  • Spectral detection
  • 3D imaging
  • Photomanipulation experiments
Products:
  • Acute brain slices
  • Cell lines
  • Ex vivo tissue slides
  • Organotypic slice culture
  • Plant tissue
  • Primary cell cultures
Access type
  • Físico
Access mode
  • Paid
Geographical availability
  • World
Languages
  • Inglês
  • Português
  • Espanhol
  • Francês
Tags:

Team

Luisa Cortes

Universidade de Coimbra

Principal Investigator Head of Microscopy Facility at CNC-UC
Service provided byLaboratório de Microscopia Ótica Avançada (LMOA)