Imaging acquisition by multiphoton microscopy

Multiphoton microscopy is an imaging methodology that allows the study of dynamic processes in cells, biological tissues and living animals without damaging them. The application of long-wavelength radiation allows deep penetration into biological materials, enabling dynamic imaging studies for example in tissue slices, in developing embryos and also in live adult animals.

The multiphoton microscope has an upright stand and is equipped with 2 infrared tunable lasers (690-1040nm), 6 visible laser lines (405/458/488/514/633 nm), 34 spectral fluorescence detectors, 2 GaAsp detectors in NDD, 2 PMT-NDD detectors in transmission and a PMT detector in transmission mode. The microscope has two stages: a motorized stage for in vitro samples and a manual stage for intravital imaging. Additionally, a perfusion system, a volatile anesthesia system and a heating mantle are available that can be attached to the microscope.